Iblot dry blotting system manual

Disassemble the device immediately after the end of the blotting procedure. 1. Open the lid of the iBlot™ Device. 2. Discard the iBlot ™Disposable Sponge and iBlot Cathode Stack, Top. 3. Carefully remove and discard the gel. Remove the transfer membrane from the stack and proceed with the denaturation step. 4. Discard the iBlot™ Anode stack, Bottom. Description of parts1. iBlot™2 Dry Blotting System User Guide The Top Stack is separated from the Bottom Stack by a white plastic separator, and contains a copper electrode and a transfer gel layer. The transfer gel layer acts as an ion reservoir and is composed of an optimized, proprietary gel composition. This manual is supplied with the iBlot 8 iBlot ® 2 Dry Blotting System. Blotting Roller. The Blotting Roller is a plastic roller attached to a stainless steel handle ( cm wide). The Blotting Roller is used to remove any air bubbles between the gel.

Perform blotting within 15 minutes of assembling the stacks with the gel. disassembling the iBlot® Gel Transfer device 1. Open the lid of the iBlot® Device. 2. Remove the iBlot® E-PAGE™ Tab (used for blotting E-PAGE™ gels only). Rinse the tab with deionized water and store in a dry place for future use. System overview The iBlot ® 2 Dry Blotting System is based on the dry blotting concept, utilizing the unique, patented gel matrix technology developed for E-Gel ® and E-PAGE ™ gels for the iBlot ® 2 Transfer Stacks. The iBlot ® 2 Transfer Stack consists of a Bottom Stack and a Top Stack. Perform blotting within 15 minutes of assembling the stacks with the gel. Assemble iBlot ™ 2 Transfer Stacks on the iBlot ™ 2 Gel Transfer Device, continued Disassemble the iBlot ™ 2 Transfer Stack. 1. Turn off the iBlot ™ 2 Gel Transfer Device. 2. Open the lid of the iBlot ™ 2 Gel Transfer Device. 3. Discard the Absorbent Pad and Top Stack. 4.

Feb iBlot 2 Dry Blotting System - Thermo Fisher Scientific. This manual is supplied with the iBlot™ 2 Gel Transfer Device (Cat. no. IB). The Invitrogen™ iBlot™ 2 Dry Blotting System is for dry electroblotting of proteins from mini-, midi- and E-PAGE gels onto nitrocellulose or PVDF membranes for. Feb While this is technically correct, the manual recommends an equilibration step of the gel in transfer buffer prior to iBlot™ transfer for more.